Gene and motif structures of MYB transcription factors
Gene structure of MYB transcription factors
In this study, a significant difference was observed in gene structure among the MYB TF members due to variation in the number and location of introns and exons. The number of introns varied from 1 to 4. A gene AtMYB41-1 has no intron in its genome which may be lost during the evolution from the ancestral sequence. Most of the exons present in the same clad were conserved. The exons present in 1st and 2nd clad showed the same position and size but a difference in intron length indicating that they are evolutionary close to each other and have similarities in functions
Conserved motif analysis of MYB transcription factors
Protein motifs of MYB TFs were analyzed by using online tool MEME (http://meme.nbcr.net/meme/cgi-bin/meme.cgi). We observed seven diverse motifs in both plant species. It was also noted that expression pattern of these motifs was diverse. However, motif 1, 2 and 3 showed regular expression patterns whereas motif 4, 5, 6 and 7 rarely showed expression across the species under study
