Gene Duplication and Chromosomal Mapping in Cajanus cajan

Gene duplication events were determined by DNAsp and the Ka/Ks ratio was calculated to determine duplication events. Divergence time was also calculated using the Ks and Ka values to predict the evolutionary events. Genomic Loci of putative K+ channels and transporters were graphically represented by the MapChart program (http://www.biometris.wur.nl/UK/Software/MapChart/download (accessed on 14 August 2021)).

Results:

In C. cajan 26 out of 39 genes were mapped on 10 chromosomes (Figure 3). The positions of thirteen genes (CcHAK13, CcHAK14, CcHAK15, CcHAK16, CcHAK17, CcKEA6, TPK-KCO4, TPK-KCO, CcSKOR, CcAKT1.2, CcKAT1.1, CcAKT1.1, and CcKAT1.2) were not found, as they were present on the scaffold. They are not represented on the map. Chromosome number eight contains a maximum number of genes (five), four transporters, and one channel. Half of the mapped genes are present on chromosomes two, eight, and eleven, while the remaining half genes are located on the remaining seven chromosomes. Chromosomes three, five, and nine contain three genes each, whereas chromosomes four, six, seven, and ten contain one gene each. There is no gene present on chromosome 1.